As part of its activities to support building public health capacities in the region and fighting infectious diseases, GHD/EMPHNET, in close coordination with the Centers for Disease Control and Prevention (CDC)/ Atlanta, conducted a training workshop on the laboratory diagnosis of brucellosis. This training targeted a group of laboratory specialists from Pakistan and focused on brucellosis diagnosis using enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (RT- PCR).
The training workshop took place between September 30 and October 4, 2018 and was hosted at the Princess Haya Biotechnology Center (PHBC) of the Jordan University of Science and Technology (JUST).
Its sessions focused on enhancing the participants’ knowledge and skills in relevance to brucellosis laboratory capacity, thus contributing to strengthened brucellosis surveillance.
The first two days of the training focused on ELISA principles, applications of these principles, and troubleshooting. They were followed by a hands-on training on the use of ELISA assays to detect anti-Brucella antibodies in milk and serum samples collected from animals.
The last three days of the training focused on laboratory biosafety as well as molecular diagnosis of brucellosis using RT- PCR. Afterwards, a practical session was conducted on the extraction of DNA from blood clot, milk, and tissue samples to detect Brucella. melitensis or abortus DNA.
This training is the second within capacity building activities held as part of a project titled Enhancing Brucellosis Surveillance in Pakistan. The project aims to increase the capacity of veterinarians working in surveillance at both field and laboratory levels.