GHD|EMPHNET Disease Control and Prevention/ Public Health Programs/ Public Health, Surveillance, Laboratory Opportunity
Evaluating the impact of enhanced laboratory-based surveillance of animal and human brucellosis in three areas of Jordan.
This proposed work intends to first, incorporate surveillance and laboratory testing that will assist to estimate and characterize the true incidence of brucellosis in humans and animals; second, identify risk factors driving transmission between animals and from animals to humans; and finally, to characterize and map the circulating Brucella species in these areas. Confirmatory testing and molecular-based methods will be established at human and animal diagnostic laboratories to align laboratory and surveillance practices with the World Health Organization (WHO) and the World Organization for Animal Health (OIE) recommendations for the identification and confirmation of human and animal brucellosis.
Active surveillance and laboratory testing including the training of veterinarians and clinicians on brucellosis signs and symptoms, collection of human and animal case data, field visits by veterinarian staff to households of confirmed brucellosis cases, and laboratory confirmation of cases at the governorate level, will be executed in year one and continue for all of the years two and three. All human and animal samples from confirmed cases will be referred and tested by Brucella spp. real-time PCR at the central veterinary, public health and RMS laboratories in years 1-3 to determine circulating species. Blood clots from confirmed human cases will be shipped to the CDC for culture and Brucella isolates recovered will undergo whole-genome sequencing (WGS) and data will be given to the Princess Haya Biotechnology Center (PHBC) at the Jordan University of Science and Technology (JUST). In year two, PHBC will be trained to conduct molecular characterization of Brucella spp. by multiple-locus variable-number repeat analysis (MLVA) on DNA extracted from PCR positive samples. PHBC will perform MLVA typing on DNA extracted from PCR positive samples collected for the duration of the study. Also, in year two, epidemiologists will be trained on methods to conduct Spatio-temporal mapping of human and animal brucellosis cases. All data will be managed in a single, relational database and will be analyzed on a quarterly basis to produce situational awareness reports to be discussed at the quarterly partners meetings.
The project’s objective is to measure the impact of active laboratory-based surveillance on the understanding of brucellosis in three endemic areas (East Amman, Karak, and Al-Mafraq) of Jordan, and to determine the true burden and major risk factors of brucellosis in humans and animals in the selected governorates. It also aims to describe the geographic distribution of the circulating Brucella sub-types that can be derived from animal and human clinical samples using non-culture-based methods, and to classify the geographic distribution of animal and human brucellosis cases in Jordan, including the distribution of different Brucella spp, is a critical component to understanding and decreasing the threat of the disease.
The project is an excellent opportunity for students to gain skills that would be beneficial for their future careers, such as public health and infectious disease surveillance, epidemiology of brucellosis and the burden of the disease, and Brucella Data collection and analysis.
During this internship, the students would be responsible for the following tasks: following-up with task owner field data collection, data entry and analysis, learning about lab specimen’s management, and testing.
This project will be based at Global Health Development, the Eastern Mediterranean Public Health Network, Amman, Jordan. Remote working options are available.
The project can begin as early as October 15 pending interest.
This is an unpaid position.
How to Apply:
Send a resume and a cover letter to Ms. Dana Shalabi: email@example.com.
Interested applicants should send their application before October 10.